On the other hand, resistant snails that did not receive BgTLR-specific siRNA but challenged with S. mansoni cleared the infection within 4 days as determined by a qPCR assay targeting the parasite glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene which failed to detect parasite product beyond day 4 (S4 Fig). This evidence concerns the gene GAPDH and infection.