We first examined the relative expression of Twist in several pancreatic cancer cell lines, namely Bxpc-3, MIA PaCa-2, Capan-1, Panc-1 and HPAC, along with HPDEC, which is a human non-malignant pancreatic duct epithelial cell line, by qRT-PCR and western bolt. Here, TWIST1 is linked to familial pancreatic carcinoma.