On account of the important role of Twist in pancreatic cancer, to indentify the pro-angiogenic effect of Twist in vivo, orthotopic xenograft tumor model was performed to compare the tumorigenesis of Bxpc-3/LV-shNC (Bxpc-3/shNC) and Bxpc-3/LV-shTwist (Bxpc-3/shTwist) cells. The gene discussed is TWIST1; the disease is familial pancreatic carcinoma.