To assess whether DGKα and ζ deficiency affected cytokine production by effector CD8 T cells during LM-OVA infection, splenocytes of OT1 T cell recipient mice 7 days after LM-OVA infection were stimulated with SIINFEKL peptide in vitro for 4-5 hours, followed by intracellular staining for TNFα and IFNγ. This evidence concerns the gene TNF and infection.