Although there was little research mentioned that C16orf45 and RASGRP3 were directly associated with CRC, our results suggested miR-1184, miR-326 and miR-330-5p were involve in CRC by interacting with TCF4, C16orf45 and RASGRP3. Therefore, it might be inferred that TCF4, C16orf45 and RASGRP3, mediated by these miRNAs, might play important roles in the CRC progression and be used as the therapeutic targets. The gene discussed is TCF4; the disease is colorectal carcinoma.