In order to further investigate the pathogenesis and explore the molecular therapeutic methods for CRC, we re-analyzed the mRNA expression of CRC tissues extracted after surgical resection at four time points and screened a set of time-related genes, such as PITX2 (paired-like homeodomain 2), VSNL1 (visinin-like 1), TCF4 (transcription factor 4), MEF2C (myocyte enhancer factor 2C) and FOS (FBJ murine osteosarcoma viral oncogene homolog). The gene discussed is PITX2; the disease is colorectal carcinoma.