At 12 h post-transfection with plasmid, which is a time prior to HeV-M nuclear egress in live infection, HeV-M was observed largely in the host cell nucleus and nucleolus in cells transfected with siNEG or siFBL and there was no discernible difference in the nuclear and nucleolar import of M between the two treatment groups (Fig 5C), where M colocalized with the nucleolar marker nucleolin. Here, NUCLEOLIN is linked to infection.