Western blotting showed that OM could reverse high glucose induced high expression of Arkadia, p-Smad2 and p-Smad3 (Fig 5A) The results of real-time PCR showed that Arkadia was increased in the HG group as compared to the NG group, and OM significantly decreased Arkadia mRNA in the HM group as compared to the HG group (Fig 5B). Here, RNF111 is linked to ocular melanoma.