ACTA1 and ocular melanoma: To determine whether OM affected related EMT marker expression levels, E-cadherin, a marker for epithelial cells, and α-SMA, a marker for mesenchymal cells, were examined by IF-LSCM and western blotting, which showed that E-cadherin was significantly reduced and α-SMA was markedly increased in the HG group as compared to the NG group.