APC and intrahepatic cholangiocarcinoma: The presence of peaks consistent with minor mutant alleles were confirmed for all the single base substitutions as well as for one out-of-frame indel on APC. As candidates of novel biomarkers, we report the recurrent nonsilent mutations (i.e., 19 nonsilent mutations observed in more than one ICC genomes but not listed in Figure 1) in Supplementary Figure S2.