Taken together, Ad-Bmi-1i selectively silence Bmi-1 expression in Bmi-1 overexpressed GC cells, and Bmi-1 knockdown by Ad-Bmi-1i not only inhibits tumor growth and stem cell-like phenotype by inducing cellular senescence directly, but also has an indirect anti-tumor activity by anti-angiogenesis effects via regulating PTEN/AKT/mTOR/VEGF pathway. The gene discussed is BMI1; the disease is gastric cancer.