INS and polycystic ovary syndrome: When PCOS subjects were divided into insulin-resistant (PCOS IR group, HOMA-IR>2.5) and non-insulin-resistant (PCOS non-IR group, HOMA-IR<2.5) groups, an increase in DCFH fluorescence with respect to controls was observed in the PCOS non-IR (p<0.05, Fig 1B and 1C) and PCOS IR (p<0.001, Fig 1B and 1C) groups, which was evidence of enhanced ROS production and, consequently, oxidative stress.