Moreover, when we subdivided the populations of PCOS patients according to tertiles of insulin (T1< 7.6, T2: 7.6–15.6, T3 >15.6 μUI/ml), we observed that those with a higher tertile of insulin presented higher levels of ROS production (T1 = 34.5±19.4, T2 = 36.4±22.5, T3 = 62.6±12.9; p = 0.017), and when we subdivided them according to tertiles of glucose (T1< 81.0, T2: 81.0–88.0, T3 >88.0 mg/dl), we observed that those with the higher tertile of glucose presented an increase of ROS production (T1 = 45.9±23.8, T2 = 34.9±19.6, T3 = 61.2±15.2; p<0.05). This evidence concerns the gene INS and polycystic ovary syndrome.