TNF and acute disseminated encephalomyelitis: To assess the role of TNF-α in this A129 ADE model, DENV2-infected mice born to DENV1-immune mothers were administered with a TNF-α blocking antibody or an isotype control at day 2 p.i. Survival and clinical symptoms were monitored and indicated that the anti-TNFα-treated mice survived throughout the experiment while the isotype control mice were moribund by day 4 p.i. (Fig 7A & 7B).