To investigate the role of RUNX2 in GC invasion and metastasis, RUNX2-overexpressing (exRUNX2) and knockdown cell lines (shRUNX2) were used, which were established from SGC7901 cells (RUNX2 low) and MGC803 cells as well as XN0422 primary GC cells (RUNX2 high), respectively (Supplementary Figure S1A–S1C). This evidence concerns the gene RUNX2 and gastric cancer.