The gene expression signature and the modulation of the EWS-Fli1′s related-pathways finally observed after EWS-Fli1 knock-down are in full agreement with the ones resulting from the BET bromodomain inhibition, reinforcing the assertion that JQ1(+) directly inhibits the Ewing Sarcoma malignant features through EWS-Fli1 signaling. Here, FLI1 is linked to Ewing sarcoma.