To conclude, our data suggest that C-terminal truncated HBx, in particular at 140 aa and 119 aa breakpoints, enhances stemness properties in vitro and induces a CD133 liver CSC subpopulation in HCC through modulating a distinct altered genomic profile involving FXR pathway and possibly drug metabolism (Figure 5C). This evidence concerns the gene NR1H4 and hepatocellular carcinoma.