Moreover, staining for the sialomucin podocalyxin decorated the luminal endothelial surface in the RIP1-TAg2:Vegfr2Y949F/Y949F tumour vasculature while RIP1-TAg2:WT tumours showed a chaotic podocalyxin staining pattern suggestive of altered apical–basal (luminal–abluminal) endothelial polarity (Fig. 4g,h,j) and therefore, disturbed vessel organization. This evidence concerns the gene PODXL and neoplasm.