MLPA analysis did not reveal any differences between DK-MG lines, other than EGFRvIII, with both lines having normal number of wild-type EGFR copies, hemizygous PTEN and NFkBIA, and CDKN2A deleted; all mutations reminiscent of brain tumors (Figure 2A) [5, 34, 35]. This evidence concerns the gene PTEN and brain neoplasm.