To identify the interaction proteins with EIF4G1 by using tandem affinity purification combined with mass spectrometry (TAP-MS) screening approach, we found that at least one protein, Ubiquitin-specific protease 10 (USP10), can directly interact with EIF4G1 in NSCLC cell lines. The gene discussed is EIF4G1; the disease is non-small cell lung carcinoma.