To study the functional role of EIF4G1 in NSCLC, we first directly silenced it by using lentiviral vector containing 2 shRNA specifically targeting EIF4G1 (EIF4G1-KD1 and KD2) to obtain stably “knock-down” H460, A549, H1299 cells. Here, EIF4G1 is linked to non-small cell lung carcinoma.