In studies analysing DNA methylation in liver tissue, we have previously shown that differential DNA methylation at particular CpG dinucleotides within the human PPARγ gene promoter can be used to stratify fibrosis severity in patients with NAFLD.21 Quantitatively, in mild fibrosis, CpG methylation density was measured at >70% rising to >80% in severe fibrosis, at the two loci examined. The gene discussed is PPARG; the disease is metabolic dysfunction-associated steatotic liver disease.