In conclusion, our study demonstrates that miR-378a-3p suppresses the activation of HSCs by targeting gli3, and that Hh-responsive Smo represses the transcriptional expression of miR-378a-3p through p65, promoting the activation of Hh and profibrotic genes by increasing the expression of the Hh target genes, Gli2 and Gli3, during liver fibrosis. Here, GLI2 is linked to Hepatic fibrosis.