In previously reported next-generation sequencing of primary AML samples, recurrent mutations in >20 different genes were demonstrated.27 In this subset of genes, we observed mutations in TP53 (P72R), TET2 (V218M and I1762V) and FLT3 (T227M) in all four cell lines (Figure 2b). This evidence concerns the gene FLT3 and acute myeloid leukemia.