A murine model of steatosis induced with a HFD increases NF-κB activity, which is associated with the elevated hepatic expression of pro-inflammatory cytokines such as TNF-α and IL-1 which are activated by ROS created by lipid peroxidation, responsible not only for promoting insulin resistance and Kupffer cell activation, but also for mediating cholesterol and triglyceride metabolism [25,26]. This evidence concerns the gene INS and steatosis.