ECHS1 and metabolic dysfunction-associated steatotic liver disease: In a murine model study, with the use of gel electrophoresis (DIGE) and MALDI-TOF techniques, 95 proteins were identified to exhibit significant changes during the development of NAFLD, whereas protein down-regulation was observed for enoyl coenzyme A hydratase (ECHS1), which catalyzes the second step of the mitochondrial β-oxidation of fatty acids, probably because of HFD-related hepatic steatosis [33].