Analysis of THP1 human monocytic cells or HCT-116 human colon carcinoma cells, which both express NOD2 endogenously, confirmed that CYLD was not involved in controlling Met1-Ub accumulation on HOIP whereas OTULIN was indispensable (Figures 1F, 1G, S1D, and S1E). Here, NOD2 is linked to colon carcinoma.