We successfully screened shRNA1 to stably down regulate the expression of TEM8 (Fig. 4A), and also found that ablation of TEM8 greatly attenuated the proliferation(Fig. 4B and Figure S3B) and induced G1 to S cell cycle inhibition(Fig. 4C,D) in osteosarcoma cells. Here, ANTXR1 is linked to osteosarcoma.