We tested our algorithm on adult eye images from 21 lines with RNA-interference (RNAi) mediated eye-specific (GMR-GAL4/UAS-RNAi) knockdown of fly orthologs of human genes associated with neurodevelopmental disorders, including dpten, kismet, dube3a, prosap, arm, caps, para, rk, nrx-1, mcph1, tpc1, and eph (Table S3). This evidence concerns the gene EPHA1 and neurodevelopmental disorder.