ANXA5 and acute lymphoblastic leukemia: Consistent with the absence of the sub-G1 cell population, an indicator apoptosis, in the cell cycle analysis, Annexin V/PI and Annexin V/7-AAD staining revealed that 0.5 μmol/L MLN4924 treatment marginally reduced survival in most T-ALL cell lines tested (except HSB2) for up to 24 hours (Figure 5).