Furthermore, since more than half of RCC sera were from RCC patients with an early stage (TNM I, 18/30 in 30 cases from test cohort and 511/805 in 805 cases from validation cohort), we further observed that the RLU of serum polyglutamylated DNAJC7 protein was 14437 ± 9642 and 13396 ± 6680 in these test and validation cohorts of patients with only early stage RCC. This evidence concerns the gene DNAJC7 and renal cell carcinoma.