To determine if apt loss of function caused the cyst cell phenotype cell-autonomously, we reduced apt specifically in CySCs and early cyst cells via c587-Gal4 and a UAS-controlled RNAi line targeting apt. Analysis in ovaries confirmed that expression of aptRNAi in follicle cells phenocopied apt loss of function mutants (Additional file 2: Figure S2C-D and [41]). This evidence concerns the gene LYPLA2P1 and cyst.