To assess the distributions of tubulin and BACE1 around amyloid deposits in our AD mouse model, we immunostained brain sections from five different 5- to 6-month-old 5XFAD mice with antibodies directed against specific isoforms of tubulin, including neuron-specific βIII-tubulin, acetylated α-tubulin, and polyglutamylated tubulin (Fig. 3a, third, fourth and fifth columns, respectively; Figs. S3, S4). The gene discussed is BACE1; the disease is Alzheimer disease.