As shown in Supplemental Figure S1, knockdown of NSUN2 in TP53-deficient human colorectal carcinoma HCT116 cells (HCT116 −/−) still reduced the levels of SHC proteins, suggesting that NSUN2 is able of regulating SHC expression in a TP53-independent manner. Here, SHC1 is linked to colorectal carcinoma.