To examine whether miR-1229 levels correlate with activation of Wnt/β-catenin signaling and GSK-3β, APC, and ICAT expression in clinical breast cancer tissue, real-time PCR was used to detect miR-1229 expression in eight fresh breast cancer tissues and western blotting was performed to detect GSK-3β, APC, and ICAT expression. This evidence concerns the gene GSK3B and breast cancer.