EGFR and neoplasm: In univariate analysis, the EGFR mutation detection rate was low when the specimen was obtained by biopsy (OR[odds ratio]: 0.78, p<0.001), or from lymph node (OR: 0.56, P<0.001) or bronchus(OR: 0.67, P<0.001), when the tumor proportion was lower than 20% (OR: 0.71, P<0.001), or when the test was performed by Sanger sequencing only (OR: 0.81, P=0.003).