Although the cell line showed a flat colony morphology that was similar to that of human ESCs, and the cells expressed pluripotent markers such as AKP, OCT4, SSEA4, TRA-1-60 and TRA-1-81, it could not form teratomas in severe combined immune deficiency (SCID) mice large enough to demonstrate the differentiation of tissues from the three germ layers. Here, POU5F1 is linked to Immunodeficiency.