Using one-way analysis of variance based on multiple permutations, we identified three miRNAs whose expression was significantly different between JAK2-mutant, CALR-mutant, MPL-mutant and triple-negative ET patients: miR-15a (P=0.045), miR-150 (P=0.001) and miR-519a (P=0.036) (Supplementary Figure 1). This evidence concerns the gene CALR and essential thrombocythemia.