TNF and neoplasm: The protein expression of TNF-α was significantly higher in animals aged > 10 years (p = 0.04) and animals with tumor time course greater than six months (p = 0.004); abundant vascularization (p = 0.01); lymph node involvement (p = 0.0007); metastasis (p = 0.02); and recurrence (p = 0.01) and animals that died during followup (p = 0.04) (Figure 6(b)).