HMGB1 and neoplasm: While mice injected with naïve HME cells contained 16.05 ng/ml of HMGB1 in the circulation, GemOE-tumor bearing mice contained 168.5 ng/ml of HMGB1 in their circulation (p = 0.00042, Figure 4D), thus supporting the above conclusion that GemOE phenotype is associated with cytoplasmic expression and/or extracellular secretion of HMGB1, in vivo.