In the present study, we analyzed different time points of infection and found that ACSL1, ACSL3, ACSL4, ACSL5 and ACSL6 were all recruited into the lumen of the Ct inclusion as early as 6 hpi (Supplementary Fig. S1) and as late as 24 hpi (Fig. 1A). This evidence concerns the gene ACSL3 and infection.