Our finding of activation of autophagy in the Crh −/−colon was not in line with our hypothesis of compromised autophagy, potentiallyunderlying the severe colitis in the Crh −/− mice.The non-targeted examination of the causative agents implicated with this phenomenonusing global and in-depth proteomics analysis confirmed the association betweenCrh deficiency and the autophagy pathway in the intestinal tissue. This evidence concerns the gene CRH and colitis.