INS and type 1 diabetes mellitus: CD4+ and CD8+ T cells able to recognize MHC class II and class I restricted epitopes of the beta-cell-associated antigens glutamic acid decarboxilase 65 (GAD65), proinsulin, islet tyrosine phosphatase (IA-2), and zinc transporter 8 [2] have been found in patients with type 1 diabetes using proliferation assays, ELISPOT, and fluorescent class I and class II MHC multimers [3].