In accordance with our novel findings regarding the role of intronic and intergenic hydroxymethylation in PCa, we chose two intronic genes—RASGEF1a and CUL2—and one intergenic gene—HINT1—for validation studies based primarily on the robustness of concordance between hMeDIP and hMeSEAL peaks, as well as lack of methylation and functionality in cancer. This evidence concerns the gene CUL2 and cancer.