Cullin 2 (CUL2) bore methylation marks overlapping hydroxymethylation peaks in RWPE-1, but not in 22Rv1; in contrast, RasGEF domain family member 1a (RASGEF1a) and histidine triad nucleotide-binding protein 1 (HINT1) were methylated in the same region in 22Rv1 (exhibiting 5hmC “loss” in cancer while not being methylated in RWPE-1). Here, HINT1 is linked to cancer.