We found that miR-203 exerted targeted inhibition of PIBF1 expression and downregulated its downstream p-Akt, its knockdown upregulated the expression of PIBF1 and p-Akt, and the restoration of PIBF1 abrogated the anti-proliferative effects of miR-203 in GC cells, suggesting that miR-203 might inhibit GC proliferation via targeting PIBF1/Akt signaling. This evidence concerns the gene AKT1 and gastric cancer.