Further ChIP-seq analysis in prostate cancer cell lines indicated multiple positive signals in the EcoRI fragment BE8-9 (chr2: 85778503–86781283) including H3K4me1 and FOXA1 in LNCaP; CTCF and FOXA1 in VCaP, and RAD21 in NCIH660. This evidence concerns the gene CTCF and prostate cancer.