According to the specific expression phase and spectrum of CK19 and GPC3 in the differentiation process of HPC toward mature hepatocyte, HCC was subclassified as CK19+/GPC3+, CK19−/GPC3+, and CK19−/GPC3−phenotypes, which roughly corresponded to HCC subtype transform from the HPC, immature hepatocyte, and terminal differentiated hepatocyte, respectively. This evidence concerns the gene GPC3 and hepatocellular carcinoma.