Using two fluorescent insulin mimetope-specific IAg7-tetramers (21G-22E- and 21E-22E-tetramer)28 we showed that NOD mice that had received the insulin mimetope and were still diabetes-free presented with significantly increased frequencies of insulin-specific Foxp3+Tregs (7.2±1.8 versus 20.2±1.7% of insulin-specific CD4+T cells, P<0.01, Supplementary Fig. 17). Here, CD4 is linked to diabetes mellitus.