Moreover, metaphase spreads of porcine osteosarcoma-derived cells displayed highly variable chromosome numbers, again similar to human data.43 As up to 50% of human osteosarcomas have alterations in the TP53 gene44 and various cytogenetic abnormalities,45 we consider that our porcine osteosarcoma model provides a valuable platform for studying such genetic changes. Here, TP53 is linked to osteosarcoma.