KRAS expression increased at each stage of cellular transformation and was the highest in poSARCO cells, likely owing to spontaneous gene amplification, as in many tumours.20 Quantitative PCR (Q-PCR) analysis revealed two KRAS copies in wild-type MSCs, MSC-PKC and MSC-PKCM, while poSARCO had six. This evidence concerns the gene PRKD1 and neoplasm.