Experiments with the thyroid cancer mouse model (BRAF-V600Ef/wt; TPO-Cre) demonstrated that cancer cells developed from TPO-expressing (i.e., at least partially differentiated) thyroid cells and showed EMT characteristics, consistent with the derivation of CSCs from differentiated thyroid cells via EMT, rather than from normal SCs (45). This evidence concerns the gene BRAF and thyroid cancer.