Regions of the Superior, superior medial, and/or medial frontal gyrus deactivations were also correlated with decreasing PHQ‐12 SS, PCS, and depression scores in our study, which has also been demonstrated in healthy volunteer studies of self‐reported anxiety.75 This change in the PFC activity may be important in future studies assessing the effect of medications in SDD, as anticipatory PFC activity can predict greater symptom improvement after 5HT3R antagonist Alosetron in IBS patients.78, 79. This evidence concerns the gene HTR3A and depressive symptom measurement.