Finally, we assessed the behavior of RICH2−/− mice using several read-outs designed to evaluate behavior related to locomotion and activity (Additional file 3: Fig. S3a-e), anxiety and depression (Additional file 3: Fig. S3e-p), ASD-like behavior (Fig. 5i, Additional file 4: Fig. S4a-g), and learning and memory (Fig. 5a-d and i, Additional file 4: Fig. S4h-m). This evidence concerns the gene ARHGAP44 and major depressive disorder.