In the current study, we checked the protein expression by Western blot, and we observed that the co-transfection of miR301 inhibitor with Akt, in breast cancer cells resulted in significant increase in total and phosphorylated p21Waf1/Cip1 and p27kip1 protein levels (Figure 3B and Supplementary Figure 1B). This evidence concerns the gene CDKN1B and breast cancer.