Next, to determine whether hepatocarcinogenesis is associated with changes in Wnt/β-catenin signaling or whether its signaling activity is positively correlated with CD133 expression and ALDH enzymatic activity, we compared the expression patterns of ALDH1, CD133, and WNT1 in several liver cancer cell lines, including HepG2, Huh7, and Hep3B cells, by real-time PCR analysis. Here, ALDH1A1 is linked to liver cancer.