To investigate the correlations between Wnt/β-catenin signaling and ALDH/CD133-positive liver CSC populations in liver cancer patients, we analyzed the expression of major Wnt/β-catenin signaling-associated components (Wnt1 and β-catenin) in ALDH- and/or CD133-positive tissue samples from HCC patients. The gene discussed is PROM1; the disease is liver cancer.