In this study, we show that individual knockdown of Sp1, Sp3 and Sp4 by RNAi in SKBR3 and MDA-MB-231 breast, A549 lung, SW480 colon, 786-O kidney, and Panc1, L3.6pL and MiaPaCa2 pancreatic cancer cell lines results in inhibition of cell growth, decreased survival, and inhibition of migration/invasion. This evidence concerns the gene SP3 and familial pancreatic carcinoma.