We compared radiation-induced upregulation of the DGKA protein in fibroblasts from patients developing fibrosis with low methylation (n=4) and in controls with high methylation (n=4) by western blot (Supplementary Figs 3 and 4), DAG kinase activity assay (Supplementary Fig. 2c) and quantitative protein mass spectrometric analysis (Supplementary Fig. 2b) confirming methylation-dependent DGKA induction and increased activity after radiation. This evidence concerns the gene DGKA and fibrosis.